Fig. 1.

Characterization of iPS cell-derived insulin-producing β-like cells. (A) Immunofluorescent staining: stage 3 on day 20. In vitro-derived β-like cells were stained with antibodies to GFP, nestin, and insulin on day 20 of stage 3. Cells were counterstained with DAPI. Nestin staining was reduced to minimal, although insulin staining persisted. (B) Gene expression profile of in vitro-derived β-like cells. Gene expression analysis by RT-PCR with various pancreatic cell markers during iPS cell-derived β-like cell differentiation. Total RNA was isolated from cells at stage 1 (S1), stage 2 (S2), and stage 3 (S3) of β-like cell development. B5-tubulin was used as an endogenous control. IAPP, islet amyloid polypeptide; iPSC, cells before differentiation.