Table 1.
RFO sugar concentrations in different tissues measured by LC/MS
Tissue type | Sucrose | Raffinose | Stachyose | Verbascose | Sum of RFOs | N |
Total dissected external fascicular phloem tissue | 60.0 ± 9.8 | 14.0 ± 2.4 | 521.0 ± 126.1 | 55.9 ± 14.5 | 651.0 ± 142.4 | 12 |
Total dissected internal fascicular phloem tissue | 43.2 ± 27.1 | N.D. | 425.9 ± 269.7 | 79.7 ± 51.4 | 548.7 ± 348.3 | 2 |
External fascicular phloem SE/CCC (by estimation) | 88.3 ± 17.5 | 6.6 ± 4.3 | 908.0 ± 224.1 | 81.1 ± 25.7 | 1139.1 ± 253.2 | 12 |
Internal fascicular phloem SE/CCC (by estimation) | 58.4 ± 48.3 | N.D. | 738.7 ± 479.5 | 123.3 ± 91.4 | 957.2 ± 619.2 | 2 |
Leaf disk | 3.1 ± 0.7 | 3.8 ± 0.6 | 9.0 ± 1.5 | 0.5 ± 0.1 | 16.5 ± 2.3 | 6 |
Phloem exudate | 0.4 ± 0.4 | 17.5 ± 10.9 | 9.5 ± 7.0 | 1.7 ± 1.7 | 29.4 ± 19.8 | 3 |
Xylem | 0.7 ± 0.3 | 0.1 ± 0.0 | 5.2 ± 2.6 | 1.5 ± 0.9 | 7.4 ± 2.8 | 4 |
The first two rows show the RFO sugar concentration, which was calculated based on the total volume of dissected external and internal fascicular phloem tissues. The third and fourth rows show the estimated RFO sugar concentration in external and internal fascicular SE/CCCs. The last three rows show RFO sugar concentrations calculated from total sample volume. The number format is mean ± SE. The unit of concentration is mM. N, number of observations; N.D., not detected.