Fig. 2.

Absence of p66 partially prevents glucose intolerance in obese p66 KO mice. (A) Fasting glycemia was recorded in 20-wk-old lean and obese mice, either proficient (WT, black columns) or deficient (KO, white columns) for p66shc. Obese mice of different p66 genotype were weight matched to avoid the confounding effect of fat mass on insulin sensitivity. N values for each group are indicated. Values are mean ± SEM. Significance was determined by 2 × 2 ANOVA followed by Tukey HSD post hoc test. (B) Glucose tolerance test (GTT) was performed on the same animals as in A, by injecting 1 g /kg glucose in peritoneum after overnight starvation. Glycemia was recorded at the indicated times. Values are mean ± SEM. Curves were compared by two-way ANOVA with repeated measures on one factor. (C) Insulin tolerance test (ITT) after 16 h starvation mice were injected with 2 U/kg body weight insulin in peritoneum. Glycemia was recorded at the indicated time points. Data are expressed as percentage of basal glycemia. Values are mean ± SEM of n mice.