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. 2010 Jul 12;107(30):13444–13449. doi: 10.1073/pnas.0913690107

Fig. 5.

Fig. 5.

Depletion of Mule inhibits TNFα-induced cell death. (A–C) WT fibroblasts (A and B), or HEK293cells (C) were transfected with control siRNA or siRNA for Mule (100 nM). Cells were treated with or without TNFα (5 ng/mL for MEFs and 20 ng/mL for HEK293 cells) plus adenovirus IκBα(AA) (200 MOI) for the indicated times. Apoptotic cell death was determined by Hoechst staining (A and C) or immunoblotting with anti-PARP antibody (B). (D) U2OS/shMule stable cells were treated with or without tetracycline (2 μg/mL) for 3 d and then treated with TNFα (20 ng/mL) plus adenovirus IκBα(AA) (200 MOI) for the indicated times. Apoptotic cell death was determined. Results are presented as mean ± SE and represent three independent experiments.