Fig. 2.

λ-phage and T7 consensus barcodes in 50% formamide. (A) λ-phage barcode at 27 °C with three example time traces (n = 13 molecules total in consensus). (B) λ-phage at 28 °C (n = 56 molecules in consensus). (C) λ-phage at 30 °C (n = 47 molecules in consensus). (D) λ-phage at 31 °C (n = 38 molecules in consensus). (E) λ-phage at 32 °C (n = 19 molecules in consensus). (F) A raw fluorescence micrograph showing λ-phage and T7 molecules side by side: It is possible to easily distinguish the two from the melting barcode. (G) T7 at 30 °C (n = 20 molecules in consensus). (H) T7 at 30.6 °C (n = 10 molecules in consensus). (I) T7 at 31.2 °C (n = 17 molecules in consensus). The example time traces have 10-s duration. The scale bar in all images is 2 μm. The error bars in the plots are standard errors on the ensemble mean (SEM), calculated as the SD of ensemble values divided by .