Table 1.
Kinetic parameters obtained from transient absorption measurements and fitting the νFe-His Raman intensity kinetics (τD)
| NO rebinding phases (from transient absorption) |
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| Protein | τ1 (amplitude) | τ2 (amplitude) | Constant | Heme iron motion τD (from νFe-His kinetics) |
| Myoglobin | 13 ps (0.40) | 148 ps (0.50) | 0.10 | 30 ± 10 ps |
| Myoglobin in 50% glycerol | 15.3 ps (0.42) | 71 ps (0.36) τ3 = 378 ps (0.21) | 0.01 | 40 ± 15 ps |
| Myoglobin H64V mutant | 10 ps (0.51) | 92 ps (0.43) | 0.06 | 30 ± 15 ps |
| Dehaloperoxidase | 14 ps (0.61) | 65 ps (0.38) | 0.01 | 7 ± 2 ps |
| Cytochrome c | 8.4 ps (0.99) | — | 0.01 | 6 ± 2 ps |
| Hemoglobin | 10.8 ps (0.74) | 61 ps (0.22) | 0.04 | 15 ± 6 ps |
Only for Mb in 50% glycerol, we found a third component.