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. 2010 Jul 14;107(31):13824–13829. doi: 10.1073/pnas.1008051107

Fig. 3.

Fig. 3.

Increase in WT1-specific IFN-γ–producing CD8+ T cells postvaccination. PBMC were restimulated using WT1 mRNA-electroporated mature DC for 1 wk. After 7 d, cultured PBMC were rechallenged using a WT1 peptide pool and assayed for intracellular IFN-γ production. Rechallenge with medium served as the negative control in all cases. Intracellular cytokine staining showed significantly higher percentages of IFN-γ–producing CD8+ T cells postvaccination compared with antigen stimulation of PBMC obtained prevaccination (P = 0.007) and compared with a medium control (P = 0.02; n = 9; insufficient cell numbers were available from patient UPN05 for culture and subsequent analysis).