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Effect of 1K1 and 1K1-NP mediated angiogenesis in vivo using zebrafish model. 1K1 or 1K1-NP were injected with growth-factor-reduced matrigel (Mgel) near the subintestinal vessel. SIVs were then stained with alkaline phosphate and visualized in bright field. (A): vehicle (Mgel); (B) empty PLGA in Mgel (C) 1K1 in Mgel, (D) 1K1-NP in Mgel and (E) 1K1-NP. (F) Graph shows morphometric quantification of the effect of treatment on angiogenesis. Data shown mean SEM ± from n = 3.
