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(A) Structure of TMP-Lumi4, a conjugate of trimethoprim covalently linked to a proprietary analog of a multidentate 2-hydroxyisophthalamide terbium chelate (Lumi4®-Tb) (36). The molecule has a molecular weight of 1879 and a net charge of -1 when complexed with terbium. (B) Normalized absorption (dotted) and emission (solid) spectra of TMP-Lumi4 (blue) and EGFP (red). The characteristically narrow terbium emission bands enable efficient spectral separation of signals.
