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. 2010 Jul 19;107(31):13942–13947. doi: 10.1073/pnas.0914026107

Fig. 1.

Fig. 1.

Identification of corticosteroids in lamprey plasma. (A) Biosynthetic pathways of corticosteroids and sex steroids. Steroidogenic enzymes are indicated on arrow labels. (B) Screening of HPLC-fractionated plasma extract with cortisol RIA and binding protein assay. AB, anticortisol antibody; CBP, cortisol binding protein. Arrows indicate where cortisol and 11-deoxycortisol standards elute. (C) Screening of HPLC-fractionated plasma extract with corticosterone RIA. Arrows indicate where corticosterone and 11-deoxycorticosterone elute. (D) Identification of 11-deoxycortisol by atmospheric pressure chemical ionization MS (APCI MS-MS) analysis. Fragmentation patterns generated from the authentic 11-deoxycortisol (inset) matched the plasma sample. (E) Identification of 11-deoxycorticosterone by APCI MS-MS analysis. Fragmentation patterns generated from the authentic 11-deoxycorticosterone (Inset) matched the plasma sample.