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. 2010 Aug 16;5(8):e12151. doi: 10.1371/journal.pone.0012151

Figure 2. Chicken Mx proteins lack activity in a THOV minireplicon system.

Figure 2

293T cells were transfected with THOV PB1, PB2, PA and NP, a plasmid encoding a luciferase THOV minireplicon and a SEAP expressing plasmid, together with pcDNA3 or a plasmid expressing the indicated Mx protein (human MxA, the MxA T103A mutant, wild type chicken Mx proteins SHK (Asn631) and 8.1 (Ser631) and their nuclear-localised counterparts SHK NLS and 8.1 NLS). 48 h post-transfection, luciferase activity was measured and is shown as relative light units (rlu). The mean (and SD) of 3 replicates is shown. * indicates significant difference (Students t-test) relative to pcDNA3 (p<0.05).