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. 2010 Aug 16;5(8):e12151. doi: 10.1371/journal.pone.0012151

Figure 3. Nuclear re-localisation of chicken Mx.

Figure 3

293T cells were transfected with pcDNA3 or plasmids expressing murine Mx1, SHK chicken Mx, or chicken Mx constructs containing the SV40 NLS at their N-termini (SHK NLS and 8.1 NLS). Panel A: 48 h post transfection, cells were stained using a species cross-reactive Mx specific monoclonal antibody (M143) followed by a FITC-conjugated anti-mouse antibody. Nuclei were counterstained with DAPI. Merged images are shown composed of overlaid images from the FITC and DAPI channels. Scale bar measures 10 um. Panel B: 48 h post transfection, cell lysates were prepared and 3 ug of total protein were loaded per sample for SDS-PAGE (7.5% resolving, 4% stacking). Western blotting was performed the anti-Mx antibody M143, followed by an anti-mouse alkaline phosphatase-conjugated antibody and detection using ECL. Lane 1: murine Mx1; Lane 2: SHK chicken Mx; Lane 3: SHK NLS. The position of the 79 kDa size marker is indicated by the arrow.