Figure 5. Pharmacological inhibition of TRPA1 abolishes the Slowly Adapting mechanical currents in IB4 negative neurons.
A. The total percentage of neurons expressing mechanically-evoked currents was unaltered in neurons treated with the TRPA1 channel inhibitor, HC-030031 (10 µM), compared to those treated with vehicle (DMSO 0.02%). B. Slowly Adapting currents were abolished in IB4 negative neurons pretreated with HC-030031 compared to those treated with vehicle (** p<0.01, Fisher's exact test). C. The magnitude of the Transient mechanical currents in either IB4 positive (right) or IB4 negative (left) neurons was not altered by pretreatment with HC-030031. Bars represent mean ± SEM.D. Example of a neuron with a Slowly Adapting current that is subsequently completely inhibited by HC-030031. E. Example of a neuron with a Slowly Adapting current, where in the presence of HC-030031, a small residual Transient current remains.
