Figure 7. PD98059 and heparinase suppress both laminar flow and interstitial flow-induced ERK1/2 activation.

Cells in 2-D or 3-D were pretreated with ERK1/2 inhibitor PD98059 (PD) or heparinase III (Hep) and then exposed to laminar flow or interstitial flow for 0 to 30 min. Cells were lysed and proteins were extracted for Western blotting. Gel panels were representative Western blots from three independent experiments, where similar results were found.