Fig. 3.

Uptake and release of mitochondrial Ca²⁺. Ca²⁺ movement across the inner mitochondrial membrane was followed spectrophotometrically with Arsenazo III as indicator. Seven-months old normotensive genetic control (WKY) and spontaneously hypertensive (SHR) rats were compared. Mitochondria (0.3 mg/ml) were energized with 10 mM succinate (▲ succ) in the presence of rotenone (not shown). Thereafter, 80 nmol of Ca²⁺ was added for several times which is indicated with arrows. Once net uptake was completed, 0.5 mM EGTA was added to sequester Ca²⁺. The graph inside the circle shows a zoom corresponding to a more detailed view of the first portion of the plots. Five separate experiments were carried out in duplicates. A representative trace is shown.