Fig. 6.

Mitochondrial activity of nitric oxide synthase (mtNOS) and cytochrome c oxidase (complex IV). Mitochondrial protein (0.3 mg/ml) were incubated in presence of different concentrations of Ca²⁺ 80, 160, 240, 320, 400, and 480 nmol in the medium as described in Section 2. mtNOS was evaluated measured the production of l-citrulline in (a) 1-month old and (b) 7-months old rats. Complex IV was evaluated measuring oxygen consumption of purified mitochondria isolated from rat brain of (c) 1-month old and (d) 7-months old WKY and SHR. WKY, Wistar Kyoto; SHR, Spontaneously hypertensive rats. Results are the means ± SEM of 3–6 separate experiments in duplicates. ^*p < 0.05, ^**p < 0.01 vs. control values and ^▲▲p < 0.01 vs. strains of same age.