Fig. 6.

Analysis of short- and long-term memory in svr and transgenic CPD mutants, using an assay based on courtship behavior. The percentage of time a male spends engaged in courtship during a test period of 10 min is referred to as the courtship index (CI) [34]. a Wild-type, svr ^poi, and svr ¹ mutants 0-min courtship levels and 60-min courtship levels are significantly suppressed (**p < 0.001, ***p < 0.0005) compared to naive courtship levels. Courtship levels are compared within genotype; the similar changes indicate that immediate recall memory and short-term memory at 0 and 60 min remain intact in the mutant flies. For naive courtship, wild-type flies n = 16, svr ^poi n = 19, svr ¹ n = 18; 0-min memory wild-type flies n = 19, svr ^poi n = 19, svr ¹ n = 20; for 60-min memory wild-type flies n = 19, svr ^poi n = 20, svr ¹ n = 20. b Comparison of long-term memory (LTM)-trained and naive-trained courtship activity. Wild-type flies show a reduction in courtship index in the LTM-trained group, relative to the naive-trained group, indicating long-term memory. In contrast, the courtship index of the svr ^poi and svr ¹ flies is comparable between the LTM-trained and naive-trained groups, indicating no long-term memory. The LTM-trained courtship levels for the wild-type, 2-3-t2, and 1B-short groups of flies are significantly suppressed compared to naive-trained courtship levels (*p < 0.05), indicating that LTM remains intact for 2-3-t2 and 1B-short transgenic CPD mutants. Wild-type flies n = 37 for naive- and LTM-trained; svr ^poi n = 26 and 23; svr ¹ n = 26 and 20; 2-3-t2 n = 19 and 16, respectively, and for 1B-short flies n = 17