Table 1B.
Verification of PAX3-FKHR Binding Site via Enhancer Assay
| Fold Induciton by | ||||||||
|---|---|---|---|---|---|---|---|---|
| Gene | Enh No. | Binding Intensity | None | PAX3 | PAX3- FKHR | Rel. Location | Dist. Tx Start bp | Dist. Tx End bp |
| Vector | None | NA | 1.0 | 1.0 | 0.9 | NA | NA | NA |
| ALK | 1 | high | 2.6 | 10.8 | 19.5 | 3rd Intron | −262,835 | 464,601 |
| FGFR4 | 1 | low | 1.5 | 1.9 | 3.7 | 3' downstream | −8,913 | −839 |
| FGFR4 | 2 | high | 1.9 | 5.7 | 8.3 | 3' downstream | −16,273 | −8,199 |
| IGF1R | 1 | Med | 1.8 | 4.0 | 4.8 | 2nd Intron | −136,375 | 171,486 |
| MET | 1 | Low | 3.9 | 10.2 | 7.4 | 3rd Intron | −35,329 | 61,711 |
| MET | 2 | High | 1.3 | 2.5 | 3.8 | 18th Intron | −83,277 | 13,763 |
| MYCN | 1 | Med | 0.9 | 3.8 | 5.4 | 3' downstream | −96,819 | −92,786 |
| MEOX1 | 1 | Med | 1.2 | 2.4 | 5.4 | 5' upstream | 994 | 20,274 |
| MEOX2 | 1 | High | 0.8 | 0.8 | 3.8 | 1 intron | −798 | 73,218 |
| PRRX1 | 1 | Med | 0.6 | 2.1 | 18.2 | 1st intron | −4,226 | 67,742 |