Table 2. : Antibodies Used for Western Blot Studies.
| Antibody | Species | Concentration | Incubation | Company |
|---|---|---|---|---|
| GluR1 | Mouse | 1 : 100 | 16 h | Santa Cruz Biotechnology Inc, Santa Cruz, CA |
| GluR2 | Mouse | 1 : 1000 | 2 h | US Biological, Swampscott, MA |
| GluR3 | Mouse | 1 : 500 | 16 h | US Biological, Swampscott, MA |
| GRIP1 | Rabbit | 1 : 1000 | 16 h | Upstate, Lake Placid, NY |
| NSF | Mouse | 1 : 4000 | 16 h | Abcam, Cambridge, MA |
| SAP97 | Mouse | 1 : 1000 | 16 h | Abcam, Cambridge, MA |
| EEA1 | Mouse | 1 : 1000 | 2 h | BD Transduction, San Jose, CA |
| PSD95 | Mouse | 1 : 1000 | 1 h | Millipore, Bellarica, MA |
| GRP78/BiP | Mouse | 1 : 250 | 1 h | BD Transduction, San Jose, CA |
| GS | Mouse | 1 : 5000 | 1 h | BD Transduction, San Jose, CA |
| Rab5 | Mouse | 1 : 2000 | 1 h | Abcam, Cambridge, MA |
| β-Tubulin | Mouse | 1 : 10,000 | 1 h | Upstate, Lake Placid, NY |
Abbreviations: EEA1, early endosome antigen 1; GluR, glutamate receptor; GRIP1, glutamate receptor-interacting protein 1, GRP78/BiP, glucose-regulated protein 78/binding protein; GS, glutamine synthetase; NSF, N-ethylmalemide sensitive factor; PSD95, postsynaptic density 95; SAP97, synapse-associated protein 97.