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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Mol Genet Metab. 2010 Jun 9;101(1):55–61. doi: 10.1016/j.ymgme.2010.05.013

Figure 2. Mammalian cell-expressed GCLC 462S enzyme has significantly decreased enzymatic activity in vitro.

Figure 2

Plasmids encoding either the 462P wild-type or 462S GCLC polymorphism were transiently transfected into MRC5-SV2 cells and incubated for 48 hours. γ-Glutamylcysteine levels in the cell media were measured using an amino acid analyzer. Bar graphs indicate proportion of γ-GC production relative to control MRC5 lysate. Graph values represent the mean ± SEM over several experiments. P-values are based on the difference between the two polymorphisms and were calculated using a paired t-test.