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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Mol Genet Metab. 2010 Jun 9;101(1):55–61. doi: 10.1016/j.ymgme.2010.05.013

Figure 4. Apoptotic MRC5-SV2 cells overexpressing 462S demonstrate increased caspase-mediated cleavage as compared to 462P wild-type.

Figure 4

A) Transfected MRC5-SV2 cells were incubated with staurosporine to induce apoptosis. α-GCLC western blot analysis was performed on lysates. Molecular weight in kD is indicated on left. B) Band signals were quantitated and cleaved:total protein ratios were calculated. Graph values indicate mean cleaved:total protein ratios ± SEM over several experiments. P-value was calculated using an unpaired t-test.