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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Bioorg Med Chem Lett. 2010 Jul 8;20(17):5191–5194. doi: 10.1016/j.bmcl.2010.07.005

Figure 2.

Figure 2

(a) Dose-dependent binding and uptake of G5-RF6.3-FI1.3 5 in KB cells. The cells were incubated with different concentrations (300, and 1000 nM per conjugate basis) of RF dendrimer 5 for 1 or 4 h in HBSS buffer. The cells were then rinsed and resuspended in PBS buffer, and fluorescence intensity was measured in a flow cytometer; (b) Dose-dependent cytotoxicity of G5-RF2.5-MTX3.9 7 and free MTX in KB cells. Cells were treated with 7 for 1 or 4 h at four different concentrations (per MTX basis) in a RF-free HBSS-BSA medium, washed and followed by incubation in a conjugate-free growth medium for 3d. The inhibition of cell growth induced was quantified by an XTT assay, which is based on the conversion of XTT to formazan by the active mitochondria of live cells.

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