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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Invest Dermatol. 2010 May 13;130(9):2211–2221. doi: 10.1038/jid.2010.123

Figure 2. Staphylococcus epidermidis induces human β-defensin (hBDs) 2 and 3 in normal human keratinocytes.

Figure 2

(a) hBD3 and (b) hBD2 expression of undifferentiated human keratinocytes stimulated by 36 μgml−1 of sterile 10 kDa ultrafiltrates from nine bacteria conditioned culture media for 24 hours. (c) The time- and (d) dose-dependent curves of hBD3 induced by sterile <10 kDa products from S. epidermidis cultured media (SECM). (e–g) Immunofluorescent staining of hBD3 induced by 36 μg ml−1 of SECM in cultured undifferentiated keratinocytes. (e) Rabbit IgG, (f) tryptic soy broth (TSB), and (g) SECM. (h) hBD3 and (i) hBD2 expression of differentiated human keratinocytes stimulated by 36 μgml−1 of sterile 10 kDa ultrafiltrates from nine bacteria conditioned culture media for 24 hours. *P<0.05; **P<0.01; P<0.001. P-values were determined using one-way analysis of variance (ANOVA) (a, b, h, and i) or two-way ANOVA (c, d). Data shown represent two independent experiments with n = 3 per group. Scale bar = 10 μm.