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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2010 Jul 7;78(1):205–212. doi: 10.1016/j.ijrobp.2010.03.023

Figure 2. RalA/B double knockdowns do not further sensitize MIA PaCa-2 cells to IR.

Figure 2

MIA PaCa-2 cells simultaneously and stably expressing shRNAs targeting RalA and RalB or NS and RalB were generated. Top, cells were analyzed by IB to confirm knockdown. Bottom, clonogenic growth assay indicates that knockdown of both Ral proteins did not result in an additional decrease in post-IR survival compared to knockdown of either protein alone. Clonogenic survival assays were performed at least three times in triplicate. Bars represent mean ± standard deviation.