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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2010 Jul 7;78(1):205–212. doi: 10.1016/j.ijrobp.2010.03.023

Figure 3. Loss of either RalA or RalB increases persistence of the DSB marker γH2AX.

Figure 3

γH2AX expression was used as a marker of DSB. Top panels, Immunoblot analysis of γH2AX levels at indicated times after administration of IR (7 Gy). Bottom panels, immunoblot analysis of the loading control, β-actin. Relative γH2AX expression was quantified by densitometry of immunoblots and normalized to β-actin. In cells in which RalA or RalB expression had been knocked down, γH2AX expression remained elevated at time points after it was restored to background levels in control (NS) cells.