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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2010 Jul 7;78(1):205–212. doi: 10.1016/j.ijrobp.2010.03.023

Figure 4. Loss of RalB but not RalA sensitizes MIA PaCa-2 cells to IR-induced apoptosis.

Figure 4

Poly (ADP-ribose) polymerase (PARP) cleavage was used to determine if IR-induced apoptotic cell death is altered by RalA or RalB knockdown. Cells were irradiated with 7 Gy and lysates were collected at the indicated time points (in hours) or after mock irradiation (M). Top panels show immunoblots probing for PARP. Cleaved PARP is marked by a grey arrowhead. Bottom panels show immunoblots of β-actin loading control. In control (NS) and RalA knockdown cells, PARP cleavage at 24 h was similar to mock (M), with most PARP cleaved by 48 h. By contrast, in RalB knockdown cells a majority of PARP was cleaved after only 24 h.