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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Cancer Res. 2010 Jul 20;70(16):6670–6681. doi: 10.1158/0008-5472.CAN-09-4471

Figure 4.

Figure 4

Myristylated Akt3 protects WM35 and WM278 cells from PLX4720-induced apoptosis in 3-D. A, WM35 and WM35 Myr-HA-Akt3 were treated with DMSO or 1 μM PLX4720 for 1 h. Cell lysates were analyzed by Western blotting for phosphoS473 Akt, total Akt, phospho ERK1/2 and total ERK1/2. B, Serum starved WM35 and WM35 Myr-HA-Akt3 cells were seeded in 3-D collagen gels and cultured in serum-free medium containing DMSO or 1 μM PLX4720. After 48 h, cells were analyzed by annexin V staining. The quantitation of mean −/+ SD from three independent experiments is shown. C, As for A, except, WM278 and WM278 Myr-HA-Akt3 were utilized. D, As for B, except, WM278 and WM278 Myr-HA-Akt3 were utilized and cells were harvested 24 h following PLX4720 treatment.