Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2011 Oct 1.

Published in final edited form as: Biochim Biophys Acta. 2010 Jul 16;1803(10):1186–1197. doi: 10.1016/j.bbamcr.2010.06.008

Fig. 4 — Expression of MHC and myogenin in HDAC7 nuclear mutants. Whole cell extracts of HDAC7 wild-type and mutants were prepared at day 0, 2, and 4. Immunoblotting was performed to detect expression of exogenous FLAG-HDAC7 (A) or total HDAC7 (B). Whole cell extracts of HDAC7 wild-type and mutants were prepared at day 0, 2, and 4. Immunoblotting was performed to detect expression of HDAC7, MHC, and myogenin (C). The relative expression levels of MHC (white column) and myogenin (shaded column) at day 4 (D) were quantified by Photoshop software. The ratio of [MHC]/[a-tubulin] and [myogenin]/[a-tubulin] of HDAC7 (WT) at day 4 was set at 1.

Fig. 4 — Expression of MHC and myogenin in HDAC7 nuclear mutants. Whole cell extracts of HDAC7 wild-type and mutants were prepared at day 0, 2, and 4. Immunoblotting was performed to detect expression of exogenous FLAG-HDAC7 (A) or total HDAC7 (B). Whole cell extracts of HDAC7 wild-type and mutants were prepared at day 0, 2, and 4. Immunoblotting was performed to detect expression of HDAC7, MHC, and myogenin (C). The relative expression levels of MHC (white column) and myogenin (shaded column) at day 4 (D) were quantified by Photoshop software. The ratio of [MHC]/[a-tubulin] and [myogenin]/[a-tubulin] of HDAC7 (WT) at day 4 was set at 1.