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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jul 16;1803(10):1186–1197. doi: 10.1016/j.bbamcr.2010.06.008

Fig. 5.

Fig. 5

The effects of HDAC7 NLS-deletion and catalytic mutation on myogenesis. C2C12 myoblasts stably expressing wild-type, mutant HDAC7 (ΔNLS), or HDAC7 (SNA) were grown to 80% confluence and induced for differentiation. Cells were fixed and stained at days 2 (A) & 4 (C) with DAPI (blue) and antibodies against muscle-specific myosin heavy chain (MHC) (red) followed by epifluorescence microscopy. Representative images from two independent clones of each stable cell lines are shown. (B) The average number of cells expressing MHC per 100 cells at day 2. (D) The average number of nuclei per myotubes at day 4. The p values of statistical t-test are shown (* p < 0.05, ** p<0.01, or *** p < 0.001). NS: not significant. (E) Expression of exogenously expressed HDAC7 (SNA) in C2C12 cells. Western blotting was performed similar to that of Figs 4A and 4B.