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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jul 16;1803(10):1186–1197. doi: 10.1016/j.bbamcr.2010.06.008

Fig. 6.

Fig. 6

Phosphorylation of class II HDACs during C2C12 differentiation. C2C12 whole cell extracts harvested at the indicated days after the induction of differentiation were prepared. Whole cell lysates were subjected to Western blotting with anti-pS178, anti-pS344, and anti-pS479 antibodies. Relative levels ([phosphorylated HDAC7 at day 4]/[phosphrylated HDAC7 at day 0]) of phosphorylated HDAC7 at S178, S344, or S479 in differentiated myotubes and undifferentiated myocytes are normalized by actin and are shown at the bottom of each lane. The experiment was repeated 3 times. A representative image is shown. The slower migrating species likely to be other members of class IIa HDACs because phospho-antibodies cross-react with other class IIa HDACs.