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. 2010 Aug 15;24(16):1709–1717. doi: 10.1101/gad.1958410

Figure 1.

Figure 1.

Generation and phenotypic characterization of p65 S276D (PD) knock-in mice. (A) PCR analysis of mouse tail DNA. Restriction enzyme digestion with EagI was performed to detect the PD mutation on p65. (B) Sequencing of p65 cDNA obtained by RT–PCR from wild-type and PD/PD homozygous mice. (C) Immunoblot analysis of p65 expression levels in wild-type and PD homozygous MEFs generated from embryos at 12.5 dpc. (D) Interaction between p65 and IκBα or IκBβ detected by immunoprecipitation in MEFs. (E) Gross appearance of wild-type and PD/PD homozygous knock-in P10 mice.