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. 2010 Aug 15;24(16):1709–1717. doi: 10.1101/gad.1958410

Figure 3.

Figure 3.

Hyperactivation of NF-κB induced by PD mutation. (A) Translocation of p65 following TNFα (10 ng/mL) or LPS (10 μg/mL) stimulation in MEFs. (B) ChIP assay was performed with untreated or TNFα-treated (2 h) MEFs using the indicated antibodies. Precipitated IL-6 κB site DNA and IκBα κB site DNA were assayed by semiquantitative PCR. (C) Differential expression of NF-κB-regulated genes in wild-type and PD/PD livers at P5 and P10. Total RNA isolated from three pairs of wild-type and PD/PD littermates was quantified by real-time RT–PCR, and was normalized to the level of GAPDH. (D) Immunostaining of TNF in wild-type and PD/PD livers at P10. Liver cross-sections were immunostained with FITC-conjugated antibody recognizing TNF. Nuclei were stained by DAPI.