Figure 4.

Phenotype of PD homozygous knock-in mice rescued by knocking out TNFR1. (A) Phenotype of PD/PD mice can be rescued by crossing with TNFR1 knockouts, but not IL6 or IL1R knockouts. Gross appearance of P10 littermates is presented. (B) H&E staining of major organs to confirm the rescue phenomenon resulted from knocking out TNFR1. Organs from P10 littermates were analyzed. Bars: panels i–iii,vii–ix, 50 μm; panels iv–vi, 20 μm. (C) H&E staining of liver in aged wild-type and PD/PD mice that lack TNFR1. Bars: panels i–iii, 50 μm; panels iv–vi, 20 μm. (D) H&E staining of the liver in one age-matched wild-type and three PD/PD TNFR1^−/− mice that died at 6 mo of age.