Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Aug 15;24(16):1787–1801. doi: 10.1101/gad.1957510

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010 by Cold Spring Harbor Laboratory Press

PMC Copyright notice

Figure 1. — Ligatin and MCT-1/DENR promote release of tRNA and mRNA from recycled 40S subunits. (A) Domain organization of Homo sapiens Ligatin, MCT-1, and DENR and their S. cerevisiae orthologs Tma64, Tma20, and Tma22. (B) Purified native Ligatin (left panel) and recombinant Ligatin, MCT-1, and DENR (right panel) resolved by SDS-PAGE. (C) Ribosomal association of [³²P]tRNA^Leu (left panel) and [³²P]MVHL-STOP mRNA (right panel) after incubation of pre-TCs assembled on MVHL-STOP mRNA with eRFs, ABCE1, eIF6, and Ligatin, assayed by SDG centrifugation. (D) Toe-print analysis of ribosomal complexes, obtained by incubating pre-TCs assembled on MVHL-STOP mRNA, with combinations of eRFs, ABCE1, eIF6, Ligatin, MCT-1, and DENR. (E) Toe-printing analysis of SDG-purified recycled tRNA/mRNA-associated 40S subunits, obtained from pre-TCs assembled on MVHL-STOP mRNA, after their incubation with ABCE1, Ligatin, MCT-1, and DENR, as indicated. Lanes C, T, A, and G depict corresponding DNA sequences. Positions of the stop codon, full-length cDNA, and toe prints corresponding to ribosomal complexes are indicated.