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. 2010 Aug 15;24(16):1787–1801. doi: 10.1101/gad.1957510

Figure 5.

Figure 5.

eIF2- and Ligatin-mediated 48S complex formation on SV 26S mRNA. (A) Secondary structure of a fragment of SV 26S mRNA comprising the 5′-UTR and the adjacent enhancer stem. (B–F) Toe-printing analysis of 48S complex formation on SV 26S mRNA in the presence of 40S subunits, aminoacylated and deacylated tRNAMeti, Ligatin, MCT-1, DENR, eIF2, and other eIFs, as indicated. AUG50 and AUG71 triplets and positions of assembled 48S complexes are indicated. Lanes C, T, A, and G depict corresponding DNA sequences.