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. 2010 Aug 15;24(16):1802–1815. doi: 10.1101/gad.1932610

Figure 6.

Figure 6.

The NSY-7-binding site predicts AWCOFF-specific expression, and is sufficient for repression in AWCON. (A) srt-45 and srt-47 are coexpressed with the AWCOFF reporter srsx-3∷mCherry, which also labels AWB (asterisks). (B) Expression of the AWCOFF markers (srsx-3, srt-45, and srt-47) and the AWCON markers (str-2, srt-26, and srt-28) in wild-type and mutant backgrounds. n > 30 for all conditions; (*) difference from wild-type at P < 0.01 (Fisher's exact test). (C) Schematic of AWCON and AWCOFF promoters showing predicted binding sites for the transcription factors CEH-36, HMBX-1, NSY-7, and DAF-3. (D, top) Schematic of odr-1∷GFP constructs inserted as single copies. [odr-1(a)] Wild-type 2.4-kb odr-1 promoter fragment; [odr-1(b)] NSY-7-binding site added to the 5′ end of the odr-1 promoter; [odr-1(c)] NSY-7-binding site added 200 bp upstream of the ATG in the odr-1 promoter. For odr-1(c), the NSY-7-binding site is inserted close to, but does not disrupt, two CEH-36-binding sites involved in driving odr-1 expression (Kim et al. 2010). (Bottom) Expression of integrated single-copy odr-1 plasmids in wild-type and nsy-7(tm3080) backgrounds. Multiple bars within the same condition represent independently integrated lines. n > 25 for all genotypes.