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. 2010 Jul 8;107(32):14229–14234. doi: 10.1073/pnas.0913574107

Fig. 2.

Fig. 2.

MiR-99b-let-7e-miR-125a cluster expression enhances long-term multilineage reconstitution. (A) Heatmap of microRNA expression profiles of hematopoietic cells. Population designation: Sca+, Lin-c−Kit−Sca+; BM, whole BM; K, Lin-c−Kit+Sca-; LKS, Lin-c−Kit+Sca+; LT, Lin-c−Kit+Sca+CD34-Flk2-; MPP, Lin-c−Kit+Sca+CD34+Flk2+; and ST, Lin-c−Kit+Sca+CD34−Flk2+. Each column represents an independent sample. Populations were sorted from pooled BM cells from multiple animals on multiple days. Red color indicates higher expression; blue for lower. (B) Bar graph of data in A for miR-125a, miR-99b, and let-7e. (C) Primed wild-type donor marrow (5FU) was transduced with retrovirus expressing either control vector or miR-99b-let-7e-miR-125a (cluster) in addition to GFP. Contribution to peripheral blood by control or cluster-transduced BM cells (GFP+) at indicated time is shown. The first time-point indicates GFP+% in the culture 2 d posttransduction. wks: weeks; mths: months. *P < 0.05. (D) Multilineage differentiation into myeloid (Mac-1+), B (B220+), and T lineages (CD3+) among GFP+ cells 5 mo posttransplantation. C and D: n = 4–7 each. Error bars indicate SD.