Fig. 3.

miR-125a enhances HSC function. The miR-99b-let-7e-miR-125a cluster or individual miRNA was transduced and transplanted as in Fig 2C. Peripheral blood contribution by transduced BM cells was analyzed 4 mo posttransplantation. (A) Representative FACS plots. (B) Quantification of data shown in A. (C) Comparison of contribution to blood formation by control or miR-125a alone (125a). The first time point (3 d) indicates GFP⁺% in the culture posttransduction. (D) Multilineage differentiation by transduced BM cells. For B to D, n = 1–5 per group per time point shown. (E) Contribution to blood formation by transduced BM cells that were cultured ex vivo for 18 d. n = 4 for control (Ctrl) and n = 5 for miR-125a (125a). *P < 0.05. (F) Serial methylcellulose colony formation in the presence of a control or miR-125a-specific antagomir. Representative fields from primary (1⁰) and secondary (2⁰) cultures. (G and H) Purified HSPCs were transduced with control or miR-125a and transplanted. Peripheral blood contribution in recipients was quantified. See SI Materials and Methods for population definition. Each animal received either (H) 100 SLAM, (G) 1,000 LKS, or 10,000 progenitors together with 2.5 × 10⁵ supporting BM cells. n = 5 except for miR-125a–transduced CMP (n = 4). Error bars reflect SD.