Fig. 1.

SsrB Cys²⁰³ is S-nitrosylated in Salmonella cultures grown in acidified NO₂⁻. (A) Salmonella were grown in EG medium, pH 5.5, in the presence of 750 μM NO₃⁻ or NO₂⁻. S-nitrosothiols were derivatized in the biotin switch assay. SsrB::3xFLAG was detected in unfractionated bacterial cytoplasmic extracts (Upper) or in affinity-purified, biotinylated fractions (Lower). (B) S-nitrosylated SsrB was determined in Salmonella strains AV0321 (ΔssrB), AV07194 (WT; ssrB::3xFLAG), or AV08171 (SNO; C203S; ssrB C203S::3xFLAG) grown for 6 h in 750 μM NO₂⁻ EG medium, pH 5.5. Representative data from two or three independent experiments are shown.