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. 2010 Jul 26;107(32):14396–14401. doi: 10.1073/pnas.1005299107

Fig. 3.

Fig. 3.

Effects of the oxidation of Cys203 on SsrB DNA binding. (A) Binding of 300 ng SsrBc to 20 fmol of its cognate ssrA promoter after treatment with 1 mM ONOO− or 25 mM iodoacetate. The unbound, biotinylated ssrA DNA probe (U) is shown for comparison. (B and D) DNA binding of the SsrBc C203D or C203S alleles. Where indicated, selected samples in D were treated with 1 mM ONOO− before binding to the DNA. (C) Circular dichroic (CD) spectra of 0.2 mg/mL SsrBc recombinant variants. Representative data from two or three independent experiments are shown.

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