Fig. 5.
The wild-type, RNS-sensitive SsrB allele provides a selective advantage in an acute model of oral salmonellosis. The virulence of wild-type (WT) Salmonella strain AV07104 or its isogenic SsrB C203S variant strain AV08171 (C203S) was investigated in acute intragastric and i.p. models of infection. (A) Survival of NRAMP1R C3H/HeN mice challenged per os (p.o.) with 107 cfu Salmonella. (B) NRAMP1R C3H/HeN mice were inoculated i.p. with 3 × 103 cfu of Salmonella. (C) The bacterial burden was recorded in mesenteric lymph nodes (MLN), liver, and spleen 5 and 10 d after C3H/HeN mice were inoculated p.o. with 107 cfu of Salmonella. (D) Gross pathological findings of C3H/HeN mice infected p.o. with Salmonella for 10 d. Liver (L) and spleen (S) are identified; white arrows indicate hepatic and splenic abscesses. (E) Effect of 500 μg/mL of the iNOS-inhibitor l-NIL administered in drinking water on survival of C3H/HeN mice infected p.o. with 107 cfu of Salmonella. Data represent two independent experiments (n =5–11 mice per group).