Fig. 4.

Direct activation of F5H by SND1. M. truncatula leaf protoplasts were first transfected with 35S:SND1 fused with GR. The protoplasts were then treated with dexamethasone (Dex) or cycloheximide (CHX) plus Dex. Luciferase activity or F5H expression (luciferase transcript level) in control samples (no Dex) is set to 1.0. (A) The 35S:SND1-GR construct. (B) Luciferase activities in protoplasts cotransfected with SND1-GR and F5H promoter-luciferase. Dex alone cannot induce F5H promoter activity, and 2 μM CHX can completely abolish luciferase activity, indicating that de novo protein synthesis is inhibited. (C) Induction of F5H gene expression. M. truncatula Actin was used as internal control. Error bars represent SE of three biological replicates.