Fig. 5.

SND1 binds directly to the F5H promoter. (A) EMSA showing direct binding between SND1 and the F5H promoter. The NAC domain of SND1 fused with MBP was expressed in E. coli, and the purified recombinant protein incubated with biotin-labeled F5H promoter. (B) Competition analysis of SND1 binding to the F5H promoter shows that P2, P3, and P4 fragments of the F5H promoter contain the binding site. Lanes are as follows: 1 ,no competitor; 2, wild type 249-bp probe; 3-8, fragments P1-P6, respectively. MBP was used as a control protein. Unlabeled competitor DNA fragments are in 200-fold molar excess relative to the labeled probes.