Fig. 6.

SRA_1 shows high specificity toward thrombin target. (A) Fluorescence measurements of target specificity of SRA_1. In comparison to the strong fluorescent signal from antisense-hybridized SRA_1 challenged with thrombin (red), we observed only limited signal in negative control experiments with NMM only (black), antisense-hybridized SRA_1 only (purple), or antisense-hybridized unselected library molecules either alone (blue) or challenged with thrombin (cyan). (B) SRA_1 duplexes challenged with 1 μM streptavidin exhibited 27% of the fluorescence signal obtained with 1 μM thrombin.