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. 2010 Jul 26;107(32):14241–14246. doi: 10.1073/pnas.1003462107

Fig. 2.

Fig. 2.

Egfr is necessary and sufficient for repressing hub formation. (A–E) Male embryonic gonads (stage 15) stained for Vas (magenta) and Egfr (green) (A), kek1 RNA (green) (B), spi RNA (green) (C), stet RNA (green) (D), and Star RNA (green) (E). Egfr was expressed in SGPs during stage 13–17. kek1 RNA was expressed in posterior SGPs during stage 14–16. spi, stet, and Star RNA were expressed in PGCs during stage 13–16, 11–16, and 13–16, respectively. (F–M) Embryonic gonads (stage 16/17) stained for Fas3 (green), Tj (red), and Vas (blue). (F) Egfrtsla/+ embryo. (G) Egfrtsla/Egfrf24 (Egfrts) embryo. (H) Embryo expressing EgfrCA in SGPs (twist-GAL4 > UAS-EgfrCA). (I) spi1/+ embryo. (J) spi1/spi1 embryo. (K) StarIIN/StarIIN embryo. (L) stet871/Df(3L)PX62 embryo. (M) sevd2; Egfrts double-mutant embryo. (Scale bar, 10 μm.) (N) The average number of Fas3-positive cells per gonad in Egfrtsla/+ (Egfr/+), Egfrts (Egfr), sevd2; Egfrts (sev, Egfr), twist-GAL4 (twi-GAL4), twist-GAL4 > UAS-EgfrCA (twi-GAL4 > UAS-EgfrCA), spi1/+ (spi/+), spi1/spi1 (spi), stet871/+ (stet/+), stet871/Df(3L)PX62 (stet), StarIIN/+ (Star/+), and StarIIN/StarIIN (Star) embryos. Error bars represent SD. The number of gonads examined in each case is shown in parentheses. Significance was calculated using the Student's t test [*P < 0.01; **P > 0.05; #P < 0.01 (compared with wild type shown in Fig. 1M)]. The average number of SGPs ± SD per gonad at stage 16/17 was 33.9 ± 2.8 in Egfrtsla/+ embryos and 35.2 ± 3.0 in Egfrts embryos (20 gonads were examined in each case). These values were not significantly different (P > 0.05, Student's t test).