Table 1.

Niche expansion is required to allocate GSCs

Males	Number of testes examined	Number of testes lacking GSCs (%)*	Significance
gcl, nos-GAL4†	200	0 (0)
gcl, nos-GAL4 > UAS-Star‡	177	90 (50.8)	P < 0.001§
nos-GAL4 > UAS-Star¶	200	0 (0)

*For counting GSCs, adult testes were stained with anti-Vas, anti-Fas3, and 1B1 antibodies. The GSCs were identified as cells that were positive for Vas, contained a single spherical spectrosome marked by the 1B1 antibody, and were associated with Fas3-positive hub cells.

^†The male flies were derived from gcl; nos-GAL4 homozygous mothers mated with wild-type males.

^‡The male flies were derived from gcl; nos-GAL4 homozygous mothers mated with UAS-Star homozygous males.

^§Significance was calculated versus gcl, nos-GAL4, and nos-GAL4 > UAS-Star by using the Fisher's exact probability test.

^¶The male flies were derived from nos-GAL4 homozygous mothers mated with UAS-Star homozygous males.