Fig. 3.

Endogenous ethylene signaling regulates FLS2 expression. (A) Western blot showing accumulation of FLS2 proteins in Col-0, ein2-5, and etr1-1 13-d-old seedlings. The fls2 mutant was used as a negative control for FLS2 detection. Thirty micrograms of total protein were loaded in each lane. Blots stained with CCB are presented to show equal loading. Similar results were observed in at least three independent experiments. (B) Quantitative RT-PCR analysis of FLS2 expression in Col-0, etr1-1, and ein2-5 untreated 13-d-old seedlings. Transcript levels are normalized to the U-box housekeeping gene At5g15400 and are presented as relative to untreated Col-0. Similar results were observed in at least three independent experiments. Statistical significance by comparison with untreated Col-0 was assessed using one-way ANOVA followed by Dunnett's test. ***P < 0.001.