ML tree for 63 taxa of Cucumis (Fig. S3 shows a ML tree of all 113 accessions) based on combined sequences from chloroplast and nuclear data (6,202 aligned nucleotides; Table S1), analyzed under a GTR + Γ model. The tree is rooted on Muellerargia. Likelihood bootstrap values of at least 75% are given at the nodes; geographic occurrence of species is color-coded (Inset); superscript letters refer to the following: T, species transferred into Cucumis (32, 33) based on DNA sequences; S, species never before sequenced; M, specimens formerly identified as Mukia maderaspatana (Cucumis maderaspatanus); and N, species new to science. Authors of old and new names appear in Table S1.