Fig. 1.
TH17 cells induce B-cell proliferation and isotype class switching in vitro. (A) TH0 or TH17 cells from 2D2 MOG35-55-specific TCR transgenic mice were cocultured with purified, CFSE-labeled CD19+ B cells in the presence of MOG35-55 (2 μg/mL). Overall survival of cultured cells was determined 3 d later by flow cytometry on the basis of their location in the forward and side scatter (Upper). Numbers in the lower right corners represent percentage of cells located within the live gate. Proliferation of B cells (CD19+CD4−7AAD−) was assessed determining CFSE dilution on day 3 (Lower). Numbers represent percentage of divided B cells. (B) Mitomycin C–treated TH0 or TH17 cells were cocultured with isolated B cells at various ratios in the presence of MOG35-55 (2 μg/mL). All conditions were plated in triplicate. On day 10 supernatant was analyzed for antibody production and Ig class switching. Graphs show mean concentration (in nanograms per milliliter) ± SEM. *P < 0.05; **P < 0.01.