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Protective antigen heptamer incubated with 1G3 monoclonal antibody produces a supercomplex. (Left) Electron micrograph of a sample of 1G3 incubated with PA63h at ∼3∶1 (antibody to heptamer) ratio. The sample was isolated from a room-temperature SEC run and suspended in a layer of uranyl acetate stain across holes in a carbon support film. (Scale bar: 50 nm.) (Right) Electron micrograph of uranyl-acetate-stained PA63h sample supported on a continuous carbon film. (Scale bar: 50 nm.)
